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KMID : 1134820110400101430
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Journal of the Korean Society of Food Science and Nutrition
2011 Volume.40 No. 10 p.1430 ~ p.1437
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Optimization of Lipase-Catalyzed Production of Structured Lipids from Canola Oil Containing Similar Composition of Triacylglycerols to Cocoa Butter
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Moon Jun-Hee
Lee Jeung-Hee
Shin Jung-Ah
Hong Soon-Tack
Lee Ki-Teak
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Abstract
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Synthesis conditions of cocoa butter equivalents were optimized using the response surface method (RSM) by interesterification of canola oil (Ca), palmitic ethyl ester (PEE), and stearic ethyl ester (StEE). The reaction was catalyzed by immobilized lipase (Lipozyme TLIM) from Thermomyces lanuginosa to produce structured lipids containing a composition of triacylglycerols similar to cocoa butter. Reaction conditions were optimized using D-optimal design with the three reaction factors of the substrate molar ratio of canola oil to palmitic ethyl ester and stearic ethyl ester (Ca : PEE : StEE＝1:1:3, 1:1.66:5, 1:2:6, 1:2.33:7, 1:3:9, X1), enzyme ratio (2∼6%, X2), and reaction time (30∼270 min, X3). The optimal conditions that minimized acyl-migration while maximizing 1-palmitoyl-2-oleoyl-3-stearoyl glycerol (POS), 1,3-distearoyl-2-oleoyl glycerol (SOS), and 1,3-dipalmitoyl-2-oleoyl glycerol (POP) were predicted, resulting in Ca : PEE : StEE＝1:3:9, 6% of enzyme ratio, and 40 min of reaction time. The reaction product of structured lipids was synthesized again under the same conditions, showing 10.43 area% of acyl-migration, 25.31 area% of POS/PSO, 19.79 area% of SOS, and 11.22 area% of POP.
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KEYWORD
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cocoa butter equivalent, canola oil, cocoa butter, acyl-migration
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